500cod标液稀释换算公式

问题描述

精选答案

计算稀释溶液的浓度的公式：

V1×M1 = V2×M2

V =V2 -V1

V1 = 稀释前体积n

V2 = 稀释后体积

M1 = 前稀释浓度

M2 =稀释后浓度

V = 合计溶剂稀释

具体的溶液的变化过程如下：

(1)取5ml的溶液加水稀释到25ml

1000μg/ml * 5ml / 25ml = 200μg/ml

(2)再由(1)取20ml稀释到25ml

200μg/ml * 20ml / 25ml = 160μg/ml

(3)再由(2 )取20ml稀释到25ml

160μg/ml * 20ml / 25ml = 128μg/ml

(4)再由(3 )取12.5ml稀释到25ml

128μg/ml * 12.5ml / 25ml = 64μg/ml

(5) 再由(4)取2ml稀释到10ml

64μg/ml * 2ml / 10ml = 12.8μg/ml

(6) 由(5)取5ml的溶液加水稀释到25ml

12.8μg/ml * 5ml / 25ml = 2.56μg/ml

(7) 由(6)取20ml稀释到25ml

2.56μg/ml * 20ml / 25ml = 2.048μg/ml

(8) 由(7 )取20ml稀释到25ml

2.048μg/ml * 20ml / 25ml = 1.6384μg/ml

(9) 由(8 )取12.5ml稀释到25ml

1.6384μg/ml * 12.5ml / 25ml = 0.8192μg/ml

其他回答

小白爱学习

cod稀释计算方法:取1ml水样，稀释至20ml.就是稀释了20倍最后的结果乘以20就行了。